We developed a next generation microfluidic platform incorporating digital and analog biomarker detection. The device performs rapid multiplexed biomarker analysis on an ultra-low volume whole blood sample. We additionally demonstrate that the microfluidic device can be controlled and readout with a cheap and portable instrument.
Piraino F., Volpetti F., Watson C. and S.J. Maerkl, ACS Nano (2015).
We present a high-throughput microfluidic platform capable of quantitating up to 384 biomarkers in 4 distinct samples by immunoassay.
Volpetti F., Garcia-Cordero J.L. and S.J. Maerkl, PLoS One (2014).
We developed a microfluidic device able to analyze 4 biomarkers in 1,024 serum samples in in parallel for a total of 4,096 assays on a single integrated device.
Garcia-Cordero J.L. and S.J. Maerkl, Lab on a Chip (2013).
We report a microfluidic device able of quantitating 4 biomarkers in 384 samples. The approach is compatible with a wide range of clinical samples including blood serum, blood plasma, as well as research samples such as cell culture medium.
Garcia-Cordero J.L., Nembrini C., Stano A., Hubbell J.A., and S.J. Maerkl, Integrative Biology (2013).
A technical demonstration of how MITOMI can be multiplexed
Garcia-Cordero J.L. and S.J. Maerkl, Chem. Commun. doi:10.1039/C2CC37740C (2013).
We demonstrate a microfluidic device capable of measuring 768 parallel kinetic rates.
Geertz M., Shore D., and S.J. Maerkl, PNAS doi:10.1073/pnas.1206011109 (2012).
Here we report the invention of a novel microfluidic mechanism (MITOMI) for measuring molecular interactions.
Maerkl S.J. and S.R. Quake, Science, 2007. 315(5809): p. 233-237 (2007).
1 granted patent and 1 patent disclosure to date.